Human SIRT1(Sirtuin 1) ELISA Kit

Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SIRT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SIRT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SIRT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SIRT1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SIRT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SIRT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SIRT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SIRT1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Description: tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Description: A sandwich quantitative ELISA assay kit for detection of Human Sirtuin 1 (SIRT1) in samples from tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Description: A sandwich quantitative ELISA assay kit for detection of Human Sirtuin 1 (SIRT1) in samples from tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Description: tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Sirtuin 1 (SIRT1) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Sirtuin 1 (SIRT1) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Sirtuin 1 (SIRT1) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Sirtuin 1 (SIRT1) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Sirtuin 1 (SIRT1) in samples from tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Description: tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Description: tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Description: tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Description: tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Description: A sandwich ELISA kit for quantitative measurement of Human SIRT1 (Sirtuin 1) in samples from Serum, Plasma, Cell supernatant

Description: A sandwich ELISA kit for detection of Sirtuin 1 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SIRT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SIRT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SIRT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SIRT1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SIRT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SIRT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SIRT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SIRT1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SIRT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SIRT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SIRT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SIRT1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SIRT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SIRT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SIRT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SIRT1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Description: Sandwich

Description: Sandwich

Description: Sandwich

Description: Sandwich

Description: Sandwich